Hosts: Vincent Racaniello, Peter Sarnow, and Bert Semler
During a trip to California, Vincent visited Peter Sarnow and Bert Semler and spoke with them about their work on internal ribosome entry, and the requirement for a cellular microRNA for hepatitis C virus replication.
Click the arrow above to play, or right-click to download TWiV #97 (66 MB .mp3, 91 minutes)
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Links for this episode:
- Eukaryotic mRNAs that might contain an IRES (PNAS)
- Modulation of HCV RNA abundance by a liver-specific microRNA (Science)
- Viral small RNAs (PLoS Pathogens)
- Bridging IRES elements to the translation apparatus (Biochim Biophys Acta)
- A nucleo-cytoplasmic SR protein functions in viral IRES mediated translation (EMBO J)
- Nuclear vs cytoplasmic routes to IRES mediated translation (Trends in Microbiology)
- Letter read on TWiV 97
Send your virology questions and comments to twiv@microbe.tv.
Hello Vincent et al.,
I enjoyed your discussion of DI particles in TWiV 97, having “cut my teeth” on DI particles in Niza Frenkel’s lab when she was at the University of Chicago. These herpesvirus DIs were generated from high multiplicity infections (MOIs) with herpes simplex viruses (HSV). Their replication followed von Magnus effect kinetics as you and Bert discussed in the episode. I was surprised to learn that poliovirus infections don’t result in the generation of DI particles unless one uses heroic measures of lengthy serial passaging at high MOIs. The diverse ways viruses make a living is a source of continual pleasure, and I love listening to your show to learn new things. You and your colleagues’ and guests’ enthusiasm for the topics under discussion is evident. Keep up the good work.
Best,
Richard Spaete