In his weekly clinical update Dr. Griffin discusses antibody response against nonpoliovirus enteroviruses, clinical presentation and virological assessment of confirmed human monkeypox virus cases in Spain, object and surface contamination with monkeypox virus, monkeypox virus infection in humans across 16 countries, clinical characteristics of ambulatory and hospitalized patients with monkeypox virus infection, compassionate use of Tecovirimat for the treatment of monkeypox infection, monitoring of Pfizer-BioNTech vaccine among children, Pfizer and BioNTech announce updated vaccine data supporting efficacy in children, direct capture of neutralized RBD enables rapid point-of-care assessment of SARS-CoV-2 neutralizing antibody titer, Pfizer and BioNTech submit application for bivalent vaccine, and Oral Nirmatrelvir and Ritonavir in non-hospitalized vaccinated patients with COVID-19.
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Links for this episode
- Antibody response against nonpoliovirus enteroviruses (ASM) 6:45
- Clinical presentation of Monkeypox cases in Spain (The Lancet) 9:49
- Object and surface contamination with Monkeypox virus infection (CDC) 12:18
- Environmental persistence of Monkeypox virus (Emerg Inf Dis) 14:24
- Monkeypox virus infection across 16 countries (NEJM) 17:42
- Clinical characteristics of hospitalization patients with Monkeypox (CMI) 21:36
- Usage of Tecovirimat for treatment of Monkeypox infection (JAMA) 22:47
- PAXLOVID patient eligibility screening checklist (FDA)
- Remdesivir fact sheet for providers (Veklury)
- Bebtelovimab fact sheet for providers (FDA)
- Monitoring of Pfizer-BioNTech vaccine among children (CDC) 26:41
- Pfizer and BioNTech announce updated vaccine data (Pfizer) 27:27
- Capture of RBD enables point of care assessment (Science Direct) 28:18
- Pfizer and BioNTech submit application for bivalent vaccine (Pfizer) 30:57
- PAXLOVID in non-hospitalized vaccinated patients 3515
- Contribute to Floating Doctors fundraiser at PWB 3915
- Dr. Griffin’s treatment guide (pdf)
- Letters read on TWiV 930 39:38
- Timestamps by Jolene. Thanks!
Intro music is by Ronald Jenkees
Send your questions for Dr. Griffin to daniel@microbe.tv
Hi Drs. Griffin and Racaniello,
First I want to thank you for you excellent scientific reports and insights week after week. I would like to make a comment regarding the antibody response to enteroviruses and the interpretation of antibody level of protection against polio. The level of antibody anti- poliovirus that correlates with protection against polio (the disease) has been well-established. The correlate of protection is the 1/4-1/8 dilution determined by neutralization test (ttps://journals.asm.org/doi/epub/10.1128/CVI.00131-10). The detailed paper from Rosenfeld et al shows the possibility that neutralizing antibodies against poliovirus can also be elicited by other enteroviruses infections. Considering these findings, it is possible that cross-neutralizing antibodies are detected using the gold standard neutralization test specific for poliovirus. But still, these antibodies neutralized poliovirus, no matter if there were elicited by poliovirus and/or other enteroviruses. Therefore, I think, the correlate of protection against polio of 1/4-1/8 dilution is still valid.
Best,
Jeronimo Cello, PhD
Department of Microbiolgy and Immunology
School of Medicine
Stony Brook University
Michael Lewis who wrote the terrific & infuriating book Premonition would be a nice choice for a non science non doctor guest. If you can get him.
Hi Drs. Griffin and Racaniello,
The article “Cross reactive antibody response against non-polioviruses” (Rosenfeld et al) shows that immunization of mice and rabbit with several enteroviruses induce antibody cross-reactive responses. The cross-reactive enterovirus antibody responses was measured by Elisa and neutralization tests. Moreover, the investigators showed that neutralizing antibodies against poliovirus can be elicited by immunization with enterovirus 68. Based on those observations, it was suggest in the TWIV episode that CDC should review the applicability or interpretation of level of protection against poliovirus since exist the possibility that part of antibody response measured by neutralization test is cross-reactive, i.e elicited by other enterovirus than poliovirus. The correlate of protection against poliovirus measured by gold standard neutralization test has well been established at 1/4-1/8 dilution (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2897268/pdf/0131-10.pdf). Rosenfeld et al., clearly showed that specific neutralization antibody response against poliovirus measured by neutralization test can be elicited not only by poliovirus but also by other enteroviruses. Therefore, I think that correlate of protection against poliovirus should stand as it is since it does not matter if the neutralizing antibodies against poliovirus was elicited by the same virus or other enterovirus.
Best, Jeronimo
Hi Drs. Griffin and Racaniello,
The article “Cross reactive antibody response against non-polioviruses” (Rosenfeld et al) shows that immunization of mice and rabbit with several enteroviruses induce antibody cross-reactive responses. The cross-reactive enterovirus antibody responses was measured by Elisa and neutralization tests. Moreover, the investigators showed that neutralizing antibodies against poliovirus can be elicited by immunization with enterovirus 68. Based on those observations, it was suggest in the TWIV episode that CDC should review the applicability or interpretation of level of protection against poliovirus since exist the possibility that part of antibody response measured by neutralization test is cross-reactive, i.e elicited by other enterovirus than poliovirus. The correlate of protection against poliovirus measured by gold standard neutralization test has well been established at 1/4-1/8 dilution (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2897268/pdf/0131-10.pdf). Rosenfeld et al., clearly showed that specific neutralization antibody response against poliovirus measured by neutralization test can be elicited not only by poliovirus but also by other enteroviruses. Therefore, I think that correlate of protection against poliovirus should stand as it is since it does not matter if the neutralizing antibodies against poliovirus was elicited by the same virus or other enterovirus.
Best, Jeronimo
ps: Sorry if you receive more than one copy of this comment. Something is wrong with my computer.