Vincent, Alan, Dick, and Raul Andino recorded TWiV live at the ASM General Meeting in Philadelphia, where they discussed increased arterial blood pressure caused by cytomegalovirus infection, restriction of influenza replication at low temperature by the avian viral glycoproteins, first isolation of West Nile virus in Pennsylvania, and current status of influenza.

Vincent, Dick, Alan, and Saul Silverstein revisit an ebolavirus needlestick accident, and discuss the role of TLR3 in formation of Negri bodies, a New England college closed by norovirus gastroenteritis, hand, foot, and mouth disease outbreak in China, and the exit of herpes simplex virus from latency by synthesis of VP16.

Vincent, Alan, and Rich Condit converse about induction of polyomavirus replication in multiple sclerosis patients treated with the MS drug Tysabri, the extent of human polyomavirus infection, selection of influenza vaccines for the 2009-10 season, cowpox virus transmission from animals to humans, vaccinia-like virus infecting humans and cattle in Brasil, and poxviruses.