This Week in Virology

TWiV 118: The virus always rings twice

January 30, 2011

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Alan, and Rich answer listener questions about vaccinia virus, fungal viruses, synthetic viruses, influenza vaccine, HeLa cells, multiplicity of infection, and much more.

Click the arrow above to play, or right click to download TWiV #118 (68 MB .mp3, 94 minutes).

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Links for this episode:

Distribution of glycoproteins on virion surface (paper 1, paper 2) – thanks, Conor!
Susceptibility of cancer cell lines to tanapox (thanks, Cheryl!)
Poxvirus family tree
Sugar, the bitter truth (YouTube) – thanks, Mary!
BBC podcast: Artificial life (thanks, Sam!)
Arthritis and influenza at CDC
Multiplicity of infection at virology blog
Virus-like particle vaccines (thanks, Sheldon!)
TWiV on Facebook
Letters read on TWiV 118

Weekly Science Picks

Alan – What you need to know about infectious disease
Rich – Bad Project (YouTube)
Vincent – Federal research center will help develop medicines

Send your virology questions and comments to twiv@microbe.tv.

TWiV 117: The Panic Virus with Seth Mnookin

January 23, 2011

Hosts: Vincent Racaniello, Rich Condit, and Seth Mnookin

Vincent and Rich converse with Seth Mnookin, author of The Panic Virus, about vaccines, autism, thimerosal, and a contagion of human unreason run wild.

Click the arrow above to play, or right click to download TWiV #117 (69 MB .mp3, 96 minutes).

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Links for this episode:

Functional HERV-K envelope protein (thanks, Jamie!)
Reverse zoonoses
Dog influenza at CDC
TWiV on Facebook
Letters read on TWiV 117

Weekly Science Picks

Rich and Vincent – The Panic Virus by Seth Mnookin

Send your virology questions and comments to twiv@microbe.tv.

TWiV 116: Cocaine, colonies, and chickens

January 16, 2011

<p”>Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Dickson, Alan, and Rich review an adenovirus-based vaccine strategy against drug addiction, a field trial of RNAi to prevent Israeli acute paralysis virus infection in honeybees, and suppression of avian influenza transmission in transgenic chickens.

Click the arrow above to play, or right click to download TWiV #116 (64 MB .mp3, 89 minutes).

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Links for this episode:

Cocaine analog coupled to disrupted adenovirus
Field application of RNAi in honeybees
Suppression of avian influenza transmission in GM chickens (EurekAlert)
Phage tailspike protein therapy
Use of phage lysins to treat bacterial infections (one and two)
Cell to cell spread of viruses (review and inhibition by tetherin)
Flock house virus replication organelle (thanks Brett!)
TWiV on Facebook
Letters read on TWiV 116

Weekly Science Picks

Dickson – Brian Deer’s investigation of Andrew Wakefield
Rich – Photographic periodic table of the elements
Alan – Year of the bat (site one and site two)
Vincent – EteRNA (NY Times article)

Send your virology questions and comments to twiv@microbe.tv.

TWiV 115: Color me infected

January 9, 2011

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Marc Pelletier

Vincent, Alan, Rich and Marc discuss the finding that a limited number of incoming herpesviral genomes can replicate and express in a cell, and controlling viral replication in Aedes aegypti with a Wolbachia symbiont.

Click the arrow above to play, or right click to download TWiV #115 (84 MB .mp3, 117 minutes).

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Links for this episode:

Replication and expression of limited numbers of incoming viral genomes
The brainbow cassette
Release of Wolbachia infected mosquitoes in Australia (story one, two)
Journal articles on Wolbachia infection of mosquitoes (Cell, PloS Pathogens)
Wolbachia discussion on TWiV 61
TWiV on Facebook
Letters read on TWiV 115

Weekly Science Picks

Marc – Homebrew bioreactor (photo, movie) – culture bottle and drive, oil-free vacuum pumps
Rich – Logitech Harmony Universal Remote
Alan – H.M.S. Challenger Reports
Vincent – Sequence of the strawberry genome and blog post by lead author

Send your virology questions and comments to twiv@microbe.tv.

TWiV 114: Ten out of ’10

January 2, 2011

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Alan, and Rich revisit ten compelling virology stories of 2010.

Click the arrow above to play, or right click to download TWiV #114 (64 MB .mp3, 88 minutes).

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Ten virology stories of 2010:

XMRV, CFS, and prostate cancer (TWiV 113, 99, 98, 94, 89, 76, 70, 65)
The ongoing saga of polio eradication (TWiV 110, 79)
Viruses interact with the miRNA/siRNA system (TWiV 108, 72)
Endogenous viruses – retro and beyond (TWiV 105, 91, 88, 65)
Dengue virus progress and new outbreak (TWiV 111, 95, 82)
Colony collapse disorder (TWiV 104)
David Baltimore (TWiV 100)
Ode to a plaque (TWiV 68)
Vaccine contamination with circovirus (TWiV 86, 77, 75)
Universal influenza vaccines (TWiV 107)

Weekly Science Picks

Rich – Elementary schoolchildren publish a science paper (original article and editorial with video) – thanks Kathy!
Alan – White-nose syndrome blog
Vincent – Headway, headlines and healthy skepticism

Send your virology questions and comments to twiv@microbe.tv.

TWiV 113: Alan Rein on XMRV

December 26, 2010

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Alan Rein

Vincent, Alan, and Rich discuss the retrovirus XMRV with retrovirologist Alan Rein of the National Cancer Institute.

Click the arrow above to play, or right-click to download TWiV #113 (55 MB .mp3, 76 minutes).

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Links for this episode:

XMRV: A new virus is prostate cancer? (Cancer Research)
Detection of XMRV in normal and prostate tumor tissue (J Inf Dis)
Reach for a scorecard (commentary, J Inf Dis)
Retrovirology papers: one, two, three, four, commentary
Integration sites of XMRV in prostate tumor DNA (J Virol, PLoS One)
TWiV on Facebook
Letters read on TWiV 113

Weekly Science Picks

Rich – Word Lens
Alan – Tetenal Press Kit
Vincent – In the pipeline and Things I won’t work with by Derek Lowe

Send your virology questions and comments to twiv@microbe.tv.

TWiV 112: Creating a killer poxvirus

December 19, 2010

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit

Vincent, Alan, and Rich review the making of a virulent poxvirus by insertion of the gene encoding IL-4, and severe 2009 H1N1 influenza due to pathogenic immune complexes.

Click the arrow above to play, or right-click to download TWiV #112 (71 MB .mp3, 98 minutes). To download, right-click or control-click on the link, then select save as.

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Links for this episode:

Expression of IL-4 makes a killer poxvirus
Additional studies on poxvirus-IL-4 recombinants
Creation of killer poxvirus could have been predicted
Interleukin regulation of Th responses
Severe pandemic H1N1 disease due to immune complexes
Unusually high influenza mortality in 18-65 years old
The complement system
TWiV on Facebook
Letters read on TWiV 112

Weekly Science Picks

Rich – The Scientist’s Top 10 Innovations 2010
Alan – Avian Vocalizations Center
Vincent – Microbial soap from Cleaner Science (thanks, Nadia!)

Send your virology questions and comments to twiv@microbe.tv.

TWiV 111: Live at Florida Gulf Coast University

December 12, 2010

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, Ed Fussell, Andrea Leal, and Amy Sargent

The TWiV crew meets with members of the Florida Keys Mosquito Control District at Florida Gulf Coast University to discuss dengue in Florida and how to control it.

Click the arrow to play, or right-click to download TWiV #111 (76 MB .mp3, 105 minutes).

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Links for this episode:

Florida Keys Mosquito Control District
Prof. Sharon Isern
Florida Gulf Coast University
CDC report on dengue in the Florida Keys
Dengue in Miami
Mosquito release in the Caymans (Oxitec press release – pdf)
Concerns about mosquito release
TWiV on Facebook – photos of TWiV 111
Letters read on TWiV 111
Video of this episode – View above or at YouTube

Weekly Science Picks

Rich – At the Bench, A Laboratory Navigator by Kathy Barker
Alan – DEEP Indonesia Underwater Photo Contest 2010 Winners
Vincent – HHMI resources for early-career scientists

Send your virology questions and comments to twiv@microbe.tv.

TWiV at FGCU

TWiV 110: CSI virology

December 5, 2010

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit

Vincent, Alan, Rich, and Dickson discuss bacteria that can utilize arsenic in place of phosphorus, the passing of Frank Fenner, polio outbreak in The Congo, solving criminal cases of HIV transmission, and classifying viruses by capsid structure.

Click the arrow to play, or right-click to download TWiV #110 (68 MB .mp3, 93 minutes).

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Links for this episode:

Bacteria that utilize arsenic (Ed Yong’s view)
Frank Fenner passes away
Smallpox and its eradication – the ‘red book’ (pdf)
Polio outbreak in The Congo (virus isolation)
Solving criminal cases with HIV evolution (press release)
Definition of paraphyly
Order to the viral universe
International Committee on Taxonomy of Viruses (ICTV)
TWiV on Facebook
Letters read on TWiV 110

Weekly Science Picks

Dickson – CDC’s West Nile virus case count for 2010
Rich – The red bees of Red Hook
Alan – Arsenic-based life at XKCD
Vincent – PLoS iPad app

Send your virology questions and comments to twiv@microbe.tv.

TWiV 109: Virologia en México

November 28, 2010

Hosts: Vincent Racaniello, Rosa Maria del Angel, and Ana Lorena Gutiérrez

Vincent visits Mexico City and speaks with Rosa Maria del Angel and Ana Lorena Gutiérrez about virology in Mexico, and their work on dengue and caliciviruses.

Click the arrow above to play, or right-click to download TWiV #109 (58 MB .mp3, 80 minutes).

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Links for this episode:

Rosa’s PNAS paper on proteins that bind poliovirus RNA
Lorena’s papers on influence of proteins that bind poliovirus RNA on tropism and attenuation
CINVESTAV
TWiV on Facebook

Weekly Science Picks

Lorena – Caliciviruses by Hansman, Jiang, and Green
Vincent – Insights into dengue virus genome replication by Alcaraz-Estrada, Yocupicio, and del Angel

Send your virology questions and comments to twiv@microbe.tv.