TWiV 470: Just a passing phage

December 3, 2017

Bacteriophage transcytosisThe TWiV ninjas reveal that bacteriophage particles rapidly move across monolayers of eukaryotic cells from different tissues.

Hosts: Vincent Racaniello, Dickson DespommierAlan DoveRich Condit, and Kathy Spindler

Click arrow to play
Download TWiV 470 (71 MB .mp3, 117  min)
Subscribe (free): iTunesRSSemail

Become a patron of TWiV!

Links for this episode

This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe   delivery service in the country. Get $30 off your first delivery and FREE SHIPPING by going to blueapron.com/twiv.

Weekly Science Picks

Kathy – Photo of 3200 year-old tree in one image
DicksonMicrosculpture
RichA Short History Of Humans And Germs
AlanPlanets app
Vincent – Laptops Are Great. But Not During a Lecture or a Meeting

Listener Picks

BaselJoint Pathology Center
JohnOpenStax

Intro music is by Ronald Jenkees.

Send your virology questions and comments to twiv@microbe.tv

Leave a Reply to Ben Padman Cancel Reply

Your email address will not be published. Required fields are marked *

3 comments on “TWiV 470: Just a passing phage

  1. Ben Padman Dec 3, 2017

    Hi there, I heard your questions about the CLEM (Correlative Light Electron Microscopy) experiment from the transcytosis paper and wanted to clear up a few things:

    1) I acquired the optical and electron images a week apart using 2 different microscopes: a Leica SP8 laser scanning confocal and a Hitachi H7500 Transmission Electron Microscope.

    2) I used a modified version of my previously published protocol (DOI: 10.1371/journal.pone.0095967)

    3) Kathy had it right: The sub-cellular locations were relocated after ultrathin sectioning of the resin embedded sample.

    4) When I started the experiment, I was really surprised by how many phages there were inside the cells… yet none of them seemed to correlate directly with the optical data. That’s when we realised that SYBR was pH sensitive. This is a problem, because endosomes are slightly acidic (~pH6 for early endosomes; ~pH5 for late endosomes). The phages were leaking SYBR almost immediately after entering the cells. Hindsight is a cruel mistress; should have used a pH resistant dye.