Sol writes:

Oh I hope I win!

I love your podcast…

I listen to all these kind of science podcasts.

Like are we there yet? Planetary radio, star talk, science Friday, etc

Dale writes:

Hi TWIM Team

I am a graduate student of medical microbiology in Port Elizabeth, South Africa.

At the moment I study antimicrobial resistance in hospital acquired infections.

I love listening to TWIM as I do my lab work. It keeps me updated, entertained, and hearing about all the amazing articles you discuss really inspires me to continue into research!

I love books and it would be awesome to add the Colour Atlas to my growing library.

Fingers crossed and thank you for all the great content you guys produce!

Kind regards,

Dale

Sabrina writes:

Dear TWIM crew,

I was SO late listening to this podcast so I know I missed the color atlas, but alas I will try anyway (there is probably already a new contest afoot).

I am a clinical laboratory scientist in central Florida and your series of podcasts were actually what triggered me to go back to school to get my license to work in the clinical lab.

Keep up the good work!

Sabrina

Melissa writes:

Hi TWIM team,

I can’t remember if you’ve already discussed this video on the TWiM, but Harvard Medical School released a timelapse video by the Kishony Lab showing bacteria adapting to the various concentrations of antibiotic on a giant agar plate with different concentrations of antibiotic.

https://www.youtube.com/watch?v=plVk4NVIUh8

We’re all looking forward to listening to more of your podcasts!  We (Pride Lab) listen to your podcasts while we work.

-Melissa

University of California, San Diego

Johnye writes:

The 2nd paper about arbitrium highlighted gaps in my knowledge and left me with unanswered questions. In fact, I needed to relisten to segments of the discussions multiple times to be clear about what I didn’t understand. May I have a review of some terms and techniques?

Making “conditioned medium” is a new term for me. Is it media that has had bacteria grown in it? How long does bacteria need to be grown to be considered “conditioned”?

What is the significance; when and why is it used?

Could you please review the significance/function of “open reading frames and end terminal peptides”; “noncoding regions” ?

Also the terms:

“Helix turn helix motif “?

“Chip sequencing”?

were new to me. Could you or your co-hosts give some background and context for those terms/techniques/uses?

And “quorum sensing” makes sense to me. Is it correct to think of it as describing a population’s behavior (growth, genetic expression) based on its own density and resources available to support its viability?

Hope my knowledge gaps are not too basic and elementary. I really became concerned when Prof Schmidt’s suggested there was a good follow-up project appropriate high school project (or was that meant for the first paper)!

As always, warmest wishes (especially at this time of year on the east coast) and many thanks. Johnye

Johnye Ballenger

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