TWiM 146: Viral arbitrium

February 24, 2017

lysis or lysogenyHosts: Vincent RacanielloElio Schaechter, and Michael Schmidt

Vincent, Elio and Michael discuss the finding of a prion in bacteria, and how communication between bacteria guides the decision between lysis and lysogeny.

Right click to download TWiM#146 (39 MB .mp3, 54 minutes)

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One comment on “TWiM 146: Viral arbitrium

  1. Johnye Ballenger Feb 26, 2017

    The 2nd paper about arbitrium highlighted gaps in my knowledge and left me with unanswered questions. In fact, I needed to relisten to segments f the discussions multiple times to be clear about what I didn’t understand. May I have a review of some terms and techniques?

    Making “conditioned medium” is a new term for me. Is it media that has had bacteria grown in it? How long does bacteria need to be grown to be considered “conditioned”?
    What is the significance; when and why is it used?

    Could you please review the significance/function of “open reading frames and end terminal peptides”; “noncoding regions” ?

    Also the terms:
    “Helix turn helix motif “?
    “Chip sequencing”?
    were new to me. Could you or your co-hosts give some background and context for those terms/techniques/uses?

    And “quorum sensing” makes sense to me. Is it correct to think of it as describing a population’s behavior (growth, genetic expression) based on its own density and resources available to support its viability?

    Hope my knowledge gaps are not too basic and elementary. I really became concered when Prof Schmidt’s suggested there was a good follow-up project appropriate high school project (or was that meant for the first paper)!

    As always, warmest wishes (especially at this time of year on the east coast) and many thanks. Johnye

    Johnye Ballenger

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