The TWiMmers get cozy with symbionts: bacteria that allow a giant shipworm to oxidize sulfur, and algae that live within salamander cells.

In recognition of National Medical Laboratory Professionals Week, Robin Patel speaks with the TWiM team about directing a clinical bacteriology laboratory, and how an observation made by a laboratory technologist lead to the finding that Ureaplasma species can cause a system metabolic disturbance, hyperammonemia.

The TWiM team speaks with Pat Schloss about assigning sequence data to operational taxonomic units, and his experience with mSphere Direct, a new way of submitting papers for publication.

Vincent, Elio, and Michael reveal what Neanderthals ate from analysis of DNA in their teeth, and new CRISPR-Cas systems found in the genomes of uncultured microbes.

The TWiM hosts reveal why phosphorus is essential for fungal brain disease, and how bacteria kill local competitors to favor the evolution of public goods cooperation.

Vincent, Elio and Michael discuss the finding of a prion in bacteria, and how communication between bacteria guides the decision between lysis and lysogeny.

At the Rocky Mountain Laboratory in Hamilton, Montana, Vincent speaks with Robert Heinzen about the work of his laboratory on Q fever and its causative microbe, Coxiella burneti.

Vincent meets up with Katy Bosio, Michael Merchlinsky, and Shilpa Gadwal at the ASM Biothreats meeting to talk about careers for scientists outside of the ivory tower.

The TWiMers discuss how changes in domestic laundering affect the removal of microorganisms, and assembly of a nucleus-like structure during viral replication in bacteria.

Vincent, Michael, and Michele explain the use of an electrochemical gradient to eliminate bacterial biofilms, and how phage susceptibility can be transferred by exchange of receptor proteins.

Vincent, Elio and Michele wind up a year of microbial podcasts with a story about the lack of resistance to a crop antifungal compound, and how a bacterium uses a molecular caliper to measure membrane thickness.

At the Hamilton, Montana Performing Arts Center, Vincent speaks with three local high school graduates and two high school teachers about how Rocky Mountain Laboratories influenced school science programs and opened up career opportunities.

The TWiM team discusses microbial DNA found on ATM machines in New York City, and how hydraulic fracturing, or fracking, alters microbial ecosystems deep in the Earth.

Highlights of the Recent Advances in Microbial Control meeting in San Diego, and expansion of a gut pathogen by virulence factors that stimulate aerobic respiration.

Design of a synchronously lysing bacterium for delivery of anti-tumor molecules in mice, and hopanoids, the lipids that live forever, brought to you by the four Microbies of TWiM.

Insight into the biology of rhinovirus C from cryo-electron microscopy, and a novel antibiotic from a commensal bacterium that grows in the human nose, from the doctors of TWiM.

Vincent, Elio, and Michele present cell division by longitudinal scission in an insect symbiont, and thermally activated charge transport in microbial nanowires.

Michael and Vincent present Spotlights, brief reviews of classic papers in the Journal of Bacteriology, and explain how a single bacterial species can reverse autism-like social deficits in the offspring of obese mice.

The TWiMers get together at ASM Microbe 2016 in Boston to speak with David and Vanessa to talk about their work on regulation of bacterial virulence in the gut by bacterial adrenergic sensors, and the physiological mechanisms that make us ill and that help us recover.

The arrival in the US of plasmid-mediated resistance to colistin antibiotics, a last line of defense against many gram-negative bacilli, and a quorum sensing system in a eukaryote are topics of this episode hosted by Vincent, Michael, and Michele.

A eukaryote without a mitochondrion, and using a phage enzyme to eliminate intracellular bacteria are two topics discussed by the TWiMers on this episode.

A deep sequencing study of commercially available probiotics, and design and synthesis of a minimal bacterial genome are the topics tackled by Vincent, Michael, and Michele on this episode of TWiM.

Vincent, Michael, and Michele reveal how a fungal protease blunts the innate immune response and promotes pathogenicity.

Harris joins Vincent, Elio, and Michael to describe multiplex automated genome engineering, a method for targeting many modifications in a population of bacterial cells.

From the ASM Biodefense and Emerging Diseases Research meeting, Vincent speaks with Rebekah and Wyndham about their work on Rift Valley Fever virus and other vector-borne pathogens, and the evolution and pathogenesis of Yersinia pestis, the agent of plague.

Vincent and Elio marvel in the finding that a phage tail-like structure from a marine bacterium stimulates tubeworm metamorphosis, and reveal Ophidiomyces as a cause of snake fungal disease.

The microbophiles investigate the ratio of bacterial to human cells in our bodies, and how placing solar panels on a bacterium enables it to carry out photosynthesis.

The TWiM team marvels over the finding of a completely nitrifying Nitrospira, and horizontal gene transfer from Wolbachia into an animal genome.

The TWiMeriti reveal a Brazilian social bee that must cultivate a fungus to survive, and diet-mediated reduction in gut colonization by Candida albicans.

Vincent visits the laboratories of Kit and Joseph Pogliano on the campus of the University of California, San Diego, where he learns about their work on the bacterial cytoskeleton, sporulation, and the effects of antibiotics on bacterial cells.

Vincent, Elio, and Michele meet with Harry Mobley, Mary O’Riordan, and Vince Young at the University of Michigan, during the designation of the Department of Microbiology and Immunology as a Milestones in Microbiology site. They discuss how the laboratory has advanced the science and teaching of microbiology, and discuss faculty work on uropathogenic E. coli, induction of stress by bacterial infection, and the gut microbiome.

Vincent meets up with Romney and Duncan at the 79th annual meeting of the Southern California branch of the American Society for Microbiology, where they talk about emerging technologies for antimicrobial susceptibility testing, and next generation sequencing and advanced molecular diagnostics.

The TWiM cohort discusses the use of antimicrobial peptides to target specific bacteria in the microbiome, and how the intracellular bacterium Wolbachia selectively kills male hosts.

The professors of TWiM discuss a University of Wisconsin plan for rescuing biomedical research in the US, and results of a clinical trial in Bangladesh of an oral cholera vaccine.

Vincent and Michael discuss the highly diverse microbiome of uncontacted Amerindians, and how the composition of human urine plays a role in the battle for iron.     Right click to download TWiM#107 (44 MB .mp3, 91 minutes). Subscribe…

Hosts: Vincent Racaniello and Michael Schmidt Guest: Katy Bosio Vincent and Michael speak with Katy Bosio about her research on pathogenesis, immunity, and vaccines against Franciscella tularensis, the causative agent of tularemia.   Right click to download TWiM#106 (55 MB .mp3, 76…

The TWiM team reviews the microbiological safety of herbs in the United Kingdom, and how a peptide from the milkweed bug binds the ribosome and inhibits bacterial protein synthesis.

The TWiM team discusses how measles vaccination protects against other infectious diseases, and links between bacterial biofilms and colon cancer.

The TWiM team is amazed by the ocelloid, and an evolutionary battle for iron between mammalian transferrin and bacterial transferrin-binding protein.

The TWiMers discuss how aroma helps disperse yeast cells on insect vectors, and evidence that MRSA is transmitted within households.

The TWiM team celebrates 100 episodes with a Talmudic question, and discussion of how a single mutation alters bacterial host tropism.

Vincent meets up with Maria, Edward, and David at the ASM Biodefense and Emerging Infections Research meeting to talk about alternative careers for scientists.

The TWiM crew ponders the question of how a bacterium finds its middle when dividing, then divulge the transfer of interbacterial antagonism genes to eukaryotes, where they may function in innate defense.

The TWiM team reveal how bacteria in a shipworm’s gills help digest wood in the gut, and an approach that identifies a new antibiotic from the soil.

Vincent meets up with Stan Maloy on the campus of San Diego State University to talk about his career in microbiology and his work as Dean of Sciences.

Vincent, Elio, and Michael discuss a symbiosis between a nitrogen-fixing cyanobacterium and a single-celled eukaryotic alga.

Vincent, Elio, and Michael reveal that a soil-dwelling nematode can recognize and respond to a bacterial quorum sensing molecule through a sensory neuron.

Vincent, Elio, Michael and Michele discuss the possible eradication of wild poliovirus type 3, and how microsporidian parasites prevent locust swarming behavior.

Vincent meets up with Laurene and David at the Annual Meeting of the Southern California Branch of the American Society for Microbiology, where they discuss how the Los Angeles County Department of Health is preparing for an outbreak of Ebola virus infection, and Cepheid’s game-changing, modular PCR system for the diagnosis of infectious diseases.

Vincent, Michele, and Michael discuss how a gene from bacteria protects a tick from plant cyanide poisoning, and enhanced transmission of Streptococcus pneumoniae by influenza virus co-infection in mice.

Michele speaks with members of the Department of Bacteriology at the University of Wisconsin – Madison, on the occasion of its designation as a Milestones in Microbiology site, where they discuss how the department has advanced the science and teaching of microbiology.

Vincent, Elio, Michael, and Michele consider whether our eating behavior is manipulated by gastrointestinal microbiota, and an aphid gene of bacterial origin whose gene product encodes a protein that is transported to an obligate endosymbiont.

In Melbourne, Australia Vincent speaks with David, Melanie, and Adam about their work on group A Streptococcus, Helicobacter pylori, and infections of Koalas with Chlamydia.

Vincent, Michael, Elio and Michele discuss how an endosymbiont betrays its aphid host to alert plant defenses, and a new immunosuppressive cell that allows infection of neonates.

Vincent, Michael, and Michele discuss how iron might disperse bacterial biofilms in carotid arterial plaques, and controlling Salmonella by modulating host iron homeostasis.

Vincent, Elio, and Michael consider a fungal pathogen of insects that acquired a gene from its host that facilitates infection, and presence of gram-negative nosocomial pathogens on community surfaces near hospitals in Brooklyn.

Vincent, Elio, Michael, and Michelle review how a pathogen promotes plant attractiveness to insect vectors, and activation of sensory neurons that modulate pain and inflammation by bacterial infection.

Vincent, Elio, Michael, and Michelle discuss a symbiosis between a bacterium and fungus that increases the virulence of oral biofilms, and the assembly of amyloid fibers, which are needed for biofilm formation.

Vincent, Elio, Michael, and Michele review how microbial virulence can be increased as a consequence of community surveillance and adaptation to macrophages.

Vincent, Elio, Michael, and Michele discuss evidence that the acellular pertussis vaccine fails to prevent infection and transmission in nonhuman primates, and the use of bacterial cytological profiling to identify pathways targeted by antibiotics.

Vincent, Elio, Michael, and Michele discuss the amazingly high level of intergenera gene exchange among haloarchaea in an Antarctic lake, and the diversity of fungi on residential surfaces and the human forehead.

Vincent, Elio, Michael, and Michele discuss the curious outer membrane vesicles of Neisseria meningitidis, and sources of Clostridium difficile infection revealed by genome sequencing.

Vincent and Michael recorded this episode at the 53rd ICAAC in Denver, where they spoke with James Gern and James Johnson about rhinoviruses and extra-intestinal pathogenic E. coli.

Vincent and Michael discuss how infection with influenza A virus disperses Streptococcus pneumoniae biofilms leading to disease, and an amazing protein chainmail in a viral capsid.

Vincent, Elio, and Michele review how horizontal gene transfer from bacteria to an insect genome enables a tripartite nested mealybug symbiosis, and how probiotic bacteria work by competing for iron in the intestine.

Vincent, Elio and Michael review how underground mycelial networks carry signals that warn neighboring plants of aphid attack, and the presence of bacteria in the human brain.

Vincent, Elio and Michael recorded this episode before an audience at the 2013 General Meeting of the American Society for Microbiology in Denver, Colorado, where they spoke with Andrew, Ferric, Suzanne, and Michelle about their research on a phage system for evading innate immunity, retractions of research papers, bacterial infections of the eye, and cytoplasmic defenses against intracellular bacteria.

Vincent, Elio and Michael discuss the finding that copper surfaces reduce microbial burden and hospital-acquired infections in the intensive care unit.

Vincent, Laura, David, Kalin and Paul get together at the Society for General Microbiology meeting in Manchester, England to talk about next-generation approaches to antimicrobial therapy.

Vincent, Michael, and Elio discuss the HIV co-receptor CCR5 as a receptor for S. aureus leukotoxin ED, and the vineyard yeast microbiome.

Vincent, Michael, and Jo discuss how subtle gender bias of science faculty favors male students, and the relationship of invasive infection and antibody orientation at bacterial surfaces.

Vincent, Michael, and Elio discuss the horizontal transfer of antibiotic resistance genes on metal surfaces, and using bacteriophage to reverse antibiotic resistance.

Vincent, Michael, and Elio meet up with Jonathan Dworkin to discuss how bacteria form spores and how they return to vegetative growth.

Hosts: Vincent Racaniello, Michael Schmidt, and Elio Schaechter Vincent, Michael, and Elio review innate immune sensing of Listeria secreted bacterial nucleic acids, and how Wolbachia enhances egg production in Drosophila. Download TWiM #45 (52 MB .mp3,…

Vincent, Michael, Elio discuss the role of prophage excision in exit of Listeria from the phagosome, and analysis of bacterial communities in saliva.

Vincent and Michael travel to San Francisco for the 52nd Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), where they meet with Bill, John, and Victor to discuss tuberculosis, monitoring infectious disease outbreaks with online data, and outside-the-box approaches to antibacterial therapy.

Vincent and Stanley meet with Waclaw Szybalski and John Kirby at Cold Spring Harbor Laboratory on the occasion of its designation as a Milestones in Microbiology site. They reminisce about how the well known laboratory has advanced the science and teaching of microbiology, and discuss John’s work on the soil dwelling, predatory myxobacteria.

Vincent, Michael, and Elio review chapters from Microbes and Evolution, a collection of short, personal essays by microbiologists.

Vincent, Jo, Michael, and Elio review an outbreak of pertussis in Washington, and how culturing can reveal rare members of the soil biosphere.

Vincent, Jo, Michael, and Elio discuss two examples of dynamic microbial symbioses that switch between mutualistic and pathogenic states.

Vincent, Michael, and Elio explore the origin of Mycoplasma pathogens of ruminants, and share their thoughts on the recent ASM General Meeting.

Vincent, Michael, and Elio review necrotizing fasciitis, and a link between surface remodeling in gram-positive and gram-negative bacteria.

Vincent, Michael, and Ivo review the requirement for segmented, filamentous bacteria for the induction of a specific type of helper T cell in the gut.

Hosts: Vincent Racaniello, Michael Schmidt, Elio Schaechter, and Rosie Redfield. Vincent, Elio and Michael speak with Rosie Redfield about her evidence that a bacterium cannot grow on arsenic instead of phosphorus. Right click to download…

Vincent, Jo, and Michael discuss an archetypal protein transport system in bacterial outer membranes, and evidence that gut microbial enterotypes might not fall into defined groups.

On episode #30 of the podcast, Vincent, Elio, and Michael review how a toxin from Burkholderia pseudomallei inhibits protein synthesis, and the role of the gut microbiome in modulating insulin resistance in mice lacking an innate immune sensor.

On episode #29 of the podcast, Vincent and Stanley review how a phage pierces the cell membrane with an iron-loaded spike, and two programmed cell death systems in E. coli.

On episode #28 of the podcast, Vincent, Michael, and Elio review how competition within a host drives virulence of Streptococcus pneumoniae, and the expanding universe of the bacterial cytoskeleton.

On episode #27 of the podcast, Vincent, Elio, and Michael review how inflammation allows Salmonella to compete with fermenting gut microbes, and a riboswitch in bacterial and Archeal species that is triggered by fluoride.

On episode #26 of the podcast, Vincent, Elio, and Michael discuss the finding of Sutterella species in the gut of autistic children, and methods for cultivating oral bacteria.

On episode #22 of the podcast This Week in Microbiology, Vincent and Michael speak with Alfred Sacchetti, MD, Chief of Emergency Services at Our Lady of Lourdes Medical Center, about microbial infections encountered in the emergency room.

On episode #21 of the podcast This Week in Microbiology, Vincent and Elio discuss an ancient symbiosis between Alphaproteobacteria and catenulid flatworms, and a toxin from Helicobacter pylori that engages the mitochondrial fission machinery to induce host cell death.

On episode #20 of the podcast This Week in Microbiology, Vincent, Michael, and Elio follow up on the outbreaks of E. coli in Germany and cholera in Haiti, then discuss genes that confer self-identity to Proteus mirabilis.

On episode #19 of the podcast This Week in Microbiology, Vincent, Michael, Elio, and Jo discuss the genome sequence of Y. pestis from victims of the Black Death, and the effect of diet on gut microbial enterotypes.

On episode #18 of the podcast This Week in Microbiology, Vincent, Michael, Elio, and Stanley explain how to make the human intestinal commensal and benign laboratory bacterium Escherichia coli K-12 into an invasive organism, and the unearthing of century-old spores in New York City.

On episode #17 of the podcast This Week in Microbiology, Vincent, Michael and Elio focus on endosymbiosis: the spread of Rickettsia in whitefiles, and a metabolic patchwork in nested symbionts of mealybugs.

Hosts: Vincent Racaniello, Michael Schmidt, Arturo Casadevall, Stuart Levy, and David Livermore On episode #16 of the podcast This Week in Microbiology, Vincent, Michael, Arturo, Stuart, and David converse about antimicrobial resistance and why most fungi do not…

On episode #15 of the podcast This Week in Microbiology, Vincent, Michael and Jo review the number of species on Earth, evidence that the 2010 Haitian cholera outbreak originated in Nepal, and how the gut microbiota influences the immune response to influenza virus infection of the lung.

On episode #14 of the podcast This Week in Microbiology, Stanley, Margaret, Michael and Elio review how the fungus Cryptococcus escapes from macrophages, and electrical conductivity in nanowires formed by the bacterium Geobacter.

On episode #13 of the podcast This Week in Microbiology, Stanley, Jo, Michael and Elio discuss how colonic microbial ecology and risk for colitis are regulated by an inflammasome, and amelioration of intestinal inflammation in mice by delivery of a probiotic-derived soluble protein to the colon.

On episode #12 of the podcast This Week in Microbiology, Vincent, Margaret, Michael and Elio review the use of photothermal nanoblades to dissect the Burkholderia intracellular life cycle, and manipulation of chromosomes in vivo for genome-wide codon replacement in E. coli.

On episode #11 of the podcast This Week in Microbiology, Vincent, Margaret, Michael and Elio review the presence of extended spectrum beta-lactamase genes in chicken meat and in humans, and a beneficial effect of Helicobacter pylori colonization on the development of allergen-induced asthma.

On episode #10 of the podcast This Week in Microbiology, Vincent, Margaret, Elio, Michael and Dickson discuss the symbiosis between the Hawaiian bobtail squid and the luminous, gram-negative bacterium Vibrio fischeri.

On episode #9 of the podcast This Week in Microbiology, Vincent, Cliff, Elio, and Michael review the outbreak of bloody diarrhea and hemolytic uremic syndrome in Germany caused by Shiga toxin-producing beansproutsEscherichia coli O104:H4.

Vincent, Michael, and Stanley recorded episode #8 of the podcast This Week in Microbiology live at the 2011 ASM General Meeting in New Orleans, with guests Andreas Baümler, Nicole Dubilier, and Paul Rainey. They spoke about how pathogens benefit from disease, symbioses between chemosynthetic bacteria and marine invertebrates, and repetitive sequences in bacteria.

On episode #7 of the podcast This Week in Microbiology, Vincent, Cliff, Elio, Margaret, and Michael discuss programmed cell death in E. coli, and the daily synthesis and degradation of enzymes needed for photosynthesis and nitrogen fixation by cyanobacteria.

On episode #4 of the podcast This Week in Microbiology, Vincent, Cliff, Margaret, and Michael review foodborne bacterial illness in the context of outbreaks associated with cantaloupes and Lebanon bologna.

On episode #3 of the podcast This Week in Microbiology, Vincent, Jo, Cliff, and Ron explore the genome analysis done in support of the Amerithrax investigation, and an insecticidal enterotoxin-deficient mutant of Bacillus thurigiensis.

On episode #1 of the podcast This Week in Microbiology, Vincent, Cliff, Michael, and Stan discuss transfer of DNA from a human host to a bacterial pathogen, and the ability of dry copper to kill bacteria on contact.